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PANA qPCR™
Description
PANA qPCR™ technology uses a sequence-specific PNA probe with a fluorescent reporter and quencher. The PNA probe is designed to hybridize specifically to a nucleotide region of the target sequence.
In the absence of target, no fluorescence is detected from the reporter due to its physical proximity to the quencher. During the annealing step, the PNA probe binds to its specific target sequence, separating the reporter and quencher such that the reporter is no longer quenched. Unlike TaqMan assays, PNA probe is not hydrolyzed during amplification because its resistant to enzyme degradation. PANA qPCR™ is a powerful tool for multiplexing, allelic discrimination experiments and quantitative analysis with high specificity and sensitivity.
PCR 반응
표적DNA 결합하여 리포터와 소광자 사이의 간격을 벌림으로써 형광신호를 방출하는 PNA 프로브 기술을 이용
리포터의 종류(파장대)를 다양하게 설정하여 한번의 PCR반응으로 여러 표적 DNA를 동시에 검출할 수 있음.
효율성 : 미량의 프로브로도 충분한 검출 효율. 소광을 위한 염기서열 조작이 불필요
안정성 : 쉽게 분해되지 않으며, DNA결합 시 높은 특이도를 나타내는 PNA 프로브
FEATURES & ADVANTAGES
- 01
It can be detected with a very small amount of probe.
- 02
Nucleotide sequence manipulation is unnecessary for extinction.
- 03
It is not easily degraded and shows high specificity when binding DNA.