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Oncology - Tissue biopsy

PNAClamp™ Mutation Detection Kit IDH2

IDH2 is frequently mutated in glioma, acute myeloid leukemia, and myelodysplastic syndromes. Mutations generally involve point mutations at the R140 and R172 residues of the protein. Mutations in IDH1 and IDH2 result in deleterious “gain of function”: instead of converting isocitrate to α-ketoglutarate, mutated IDH1 or IDH2 converts isocitrate to 2-hydroxyglutarate. 2-hydroxyglutarate inhibits other proteins involved in epigenetic regulation.
CAT NO.
PNAC-5101

DETAILS


 

Total 11 IDH2 mutations can be detected.

 

 Murations

Size 

 Codon 140

Codon 172 

25 tests 

 4

 

FEATURES

  • 01High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
  • 02Real-Time PCR based ready-to-use kit
  • 03Short running time (within 3 hours)

PROCEDURE

  • sample preparation
  • Extraction of Total DNA
  • Amplification of target DNA by Real-Time PCR
  • Analysis of Result

COMPATIBLE SAMPE TYPE

- FFPE tissue
- Biopsy tissue

COMPATIBLE Real-Time PCR machine

  • CFX96
(Bio-Rad)
의 이미지

    CFX96
    (Bio-Rad)

  • LightCycler 480II
(Roche)
의 이미지

    LightCycler 480II
    (Roche)

  • ABI7500
(Thermo Fisher Scientific)
의 이미지

    ABI7500
    (Thermo Fisher Scientific)

  • ABI7900HT
(Thermo Fisher Scientific)
의 이미지

    ABI7900HT
    (Thermo Fisher Scientific)

  • StepOnePlus
(Thermo Fisher Scientific)
의 이미지

    StepOnePlus
    (Thermo Fisher Scientific)

  • QuantStudio 5
(Thermo Fisher Scientific)
의 이미지

    QuantStudio 5
    (Thermo Fisher Scientific)

  • Rotor-Gene Q
(Qiagen)
의 이미지

    Rotor-Gene Q
    (Qiagen)

‘*’ marked information should be submitted.