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Oncology - Tissue biopsy
PNAClamp™ Mutation Detection Kit IDH2
IDH2 is frequently mutated in glioma, acute myeloid leukemia, and myelodysplastic syndromes. Mutations generally involve point mutations at the R140 and R172 residues of the protein. Mutations in IDH1 and IDH2 result in deleterious “gain of function”: instead of converting isocitrate to α-ketoglutarate, mutated IDH1 or IDH2 converts isocitrate to 2-hydroxyglutarate. 2-hydroxyglutarate inhibits other proteins involved in epigenetic regulation.
CAT NO.
PNAC-5101
DETAILS
Total 11 IDH2 mutations can be detected.
Murations | Size | |
Codon 140 | Codon 172 | 25 tests |
4 | 7 |
FEATURES
- 01High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
- 02Real-Time PCR based ready-to-use kit
- 03Short running time (within 3 hours)
PROCEDURE
- sample preparation
- Extraction of Total DNA
- Amplification of target DNA by Real-Time PCR
- Analysis of Result
COMPATIBLE SAMPE TYPE
- FFPE tissue
- Biopsy tissue
- Biopsy tissue
COMPATIBLE Real-Time PCR machine
-
CFX96
(Bio-Rad)
-
LightCycler 480II
(Roche)
-
ABI7500
(Thermo Fisher Scientific)
-
ABI7900HT
(Thermo Fisher Scientific)
-
StepOnePlus
(Thermo Fisher Scientific)
-
QuantStudio 5
(Thermo Fisher Scientific)
-
Rotor-Gene Q
(Qiagen)