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PNAClamp™

Description

PNAClamp™ technology is the PNA-based PCR clamping that selectively amplifies only the mutated target DNA sequence as a minor portion in the mixture with the major wild type DNA sequences. PNAClamp™ technology takes full advantages of PNA probes that have strong binding affinity, specificity to its complementary strands and property of not being recognized at all by DNA polymerase as primer.

PNAClamp™ technology can be easily applied to detect only the mutated DNA sequences from the mixture with the wild type DNA sequences by PCR amplification. The wild type DNA fragments cannot be amplified by the PNA probes that are tightly bound, whereas only the mutated DNA sequences can be amplified selectively by PCR amplification.

FEATURES & ADVANTAGES

Accurate detection of 1% mutations in 50 ng of normal cells

The mutated DNA can be selectively amplified and detected using PNA clamping technology. It is a technology that can detect small amount of DNA with high sensitivity of 1%.

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Comparison data between PNAClamp™ and D-sequencing

  • - Using FFPE tissue from advanced NSCLC patients
  • - Higher detection rate of PNAClamp™ (PNAClamp™ 34%, D-Sequencing 26%)
  • - Low rate of non-deterministic data (within 2%)

Mutation detection on various specimens

Comparison data with D-Sequencing on various specimens [Exon21 c.2573T > G(L858R) mutant]: PNAClamp™ can detect mutations with higher sensitivity using FFPE tissue, pleural effusion, and cytology samples

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