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Oncology - Tissue biopsy
PNAClamp™ Mutation Detection Kit BCR-ABL
CML (Chronic myelogenous leukemia) patients who has consistently treated with Imatinib (Gleevec), have acquired point mutations in tyrosine kinase domain of BCR-ABL fusion gene. Especially, T315I mutation is known as a mutation associated with Imatinibresistance and observed with high frequency.
It is important to monitor patients treated with Imatinibby BCR-ABLfusion gene mutation test to prevent therapy failure due to drug resistance by mutation.
It is important to monitor patients treated with Imatinibby BCR-ABLfusion gene mutation test to prevent therapy failure due to drug resistance by mutation.
CAT NO.
PNAC-7001
DETAILS
BCR-ABL T315I can be detected by PNAClamp™ BCR-ABL Mutation Detection Kit.
Mutations | Size |
Exon 6 | 25 tests |
T315I (944C) |
FEATURES
- 01High sensitivity and specificity even with small amount of DNA(1% LOD with 10 ng wild/mutant type mixed DNA)
- 02Real-Time PCR based ready-to-use kit
- 03Short running time (within 3 hours)
PROCEDURE
- sample preparation
- Extraction of Total DNA
- Amplification of target DNA by Real-Time PCR
- Analysis of Result
COMPATIBLE SAMPE TYPE
- Whole Blood
COMPATIBLE Real-Time PCR machine
-
CFX96
(Bio-Rad)
-
LightCycler 480II
(Roche)
-
ABI7500
(Thermo Fisher Scientific)
-
ABI7900HT
(Thermo Fisher Scientific)
-
StepOnePlus
(Thermo Fisher Scientific)
-
QuantStudio 5
(Thermo Fisher Scientific)
-
Rotor-Gene Q
(Qiagen)