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Oncology - Tissue biopsy
PNAClamp™ Mutation Detection Kit BRAF
BRAF mutation is found in several cancers including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer. In case of thyroid cancer, there are over 40 different types of BRAF mutation. Of these mutations, the V600E mutation on exon 15, i.e. change of nucleotide from T to A in 1799th base that results in change from valine to glutamate in amino acids, is most important with over 90% of frequency. Especially, BRAF V600E mutation is detected with ca. 45% of frequency in papillary thyroid carcinoma and is considered a prognostic maker for thyroid cancer.
CAT NO.
PNAC-2001
DETAILS
Total 5 BRAF mutations can be detected.
Mutation | Size |
---|---|
p.V600E, p.V600D, p.V600K, p.V600R, p.V600A | 50 tests |
FEATURES
- 01High sensitivity and specificity even with small amount of DNA (1% LOD with 10 ng wild/mutant type mixed DNA)
- 02Real-Time PCR based ready-to-use kit
- 03Short running time (within 3 hours)
PROCEDURE
- sample preparation
- Extraction of Total DNA
- Amplification of target DNA by Real-Time PCR
- Analysis of Result
COMPATIBLE SAMPE TYPE
- FFPE tissue
- Fresh tissue
- Fresh tissue
COMPATIBLE Real-Time PCR machine
-
CFX96
(Bio-Rad)
-
LightCycler 480II
(Roche)
-
ABI7500
(Thermo Fisher Scientific)
-
Rotor-Gene Q
(Qiagen)