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PANAMutyper™
Description
PANAMutyper™ is a novel technology that integrates PNAClamp™ and PANA RealTyper™. PNAClamp™ is a kind of PCR technology and uses PNA (Peptide Nucleic Acid) probes which bind to the wild-type DNA complementarily. These PNA probes restrain the amplification of wild-type DNA so small amount of mutant-type DNA is selectively amplified. It makes possible to detect somatic mutation. PANA RealTyper ™ is Multiplex Melting Curve Analysis using fluorescence labeled PNA probe. PANAMutyper™ takes advantages from both technologies. It is not only able to detect small amount of mutation with high sensitivity, but also able to genotype multiple mutations simultaneously using the phenomena that has their own melting temperature(Tm) value for the sequence changes of the target gene.
PNAClamp™ + PANA RealTyper™
FEATURES & ADVANTAGES
Higher sensitivity (LOD < 0.1%)
Mutant-type DNA is selectively amplified by PNAClamp™. PANAMutyper™ is suitable to liquid biopsy which needs high sensitivity.
*PNAClamp™: This technology is based on the strong binding affinity of PNA and the fact that PNA cannot be used as a PCR primer. A PNA clamping probe binds to the wild-type DNA and suppresses the amplification of the wild-type DNA. And then, only mutant-type DNA is selectively amplified.
Multiplex Genotyping
The selectively amplified target mutant-type DNA is genotyped by PANA RealTyper™(Melting Curve Analysis).
PANA RealTyper™: Fluorescently labeled PNA probes fluoresce only when bound to the target sequence. PNA probes with a unique melting temperature (Tm) according to the nucleotide sequence are denatured from the target DNA at the Tm as the temperature increases and the fluorescence signal decreases. Genotyping of the target DNA is possible by analyzing the temperature at which the signal decreases (melting temperature).
Applications
It’s possible to detect ctDNA (circulating tumor DNA) only with minute amount of blood sample by using this technology while previous cancer diagnosis method is surgical and invasive way to take out tumor tissue. This product is a breakthrough in molecular diagnosis, since it’s convenient to use and detect mutated genes in ctDNA within 3 hours. This method is expected to be applicable to diagnose early detection of various cancers. Moreover, it will help disease diagnosis and treatment as well as predicting the prognosis.
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