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Oncology - Tissue biopsy

PNAClamp™ Mutation Detection Kit PIK3CA

PIK3CA mutation activates PI3K (Phosphatidylinositol 3-kinase) protein and PI3K activates several signaling pathways of tyrosine kinase receptors. Activated tyrosine kinase represses the function and excitometabolism of protein which inhibits cell survival and division. These abnormal changes initiate the process of cancer development by activating the protein involved in cell growth. Point mutations in PIK3CA are detected with ca. 80% of frequency in exon 9 (interpreted as helical part of PI3K protein) and exon 20 (interpreted as kinase part), while other types of mutations are also seen in many different locations. PIK3CA mutations are found with 25 to 40 % frequency in various types of cancer like colorectal cancer, gastric cancer, lung cancer, brain cancer, endometrial cancer, ovarian cancer, breast cancer, and more.

PNAClamp™ PIK3CA Mutation Detection Kit utilizes modified PCR technology, using optimized PNA probes that tightly bind to wild type DNA templates. This tight binding to the wild type DNA templates result in no amplification of the wild type DNA template in PCR reaction while the mutated genes, especially with SNPs, PCR amplification is processed for multiplication of the mutated DNA sequences.
CAT NO.
PNAC-4001

DETAILS

 


 

Total 15 PIK3CA mutations can be detected.

 

 Mutations

 Size

 Codon 542

 Codon 545

 Codon 546

 Codon 1047

Codon 420

 25 tests

 3

 1

 

FEATURES

  • 01High sensitivity and specificity even with small amount of DNA
  • 02Real-Time PCR based ready-to-use kit
  • 03Short running time (within 3 hours)

PROCEDURE

  • sample preparation
  • Extraction of Total DNA
  • Amplification of target DNA by Real-Time PCR
  • Analysis of Result

COMPATIBLE SAMPE TYPE

- FFPE tissue
- Plasma

COMPATIBLE Real-Time PCR machine

  • CFX96
(Bio-Rad)
의 이미지

    CFX96
    (Bio-Rad)

  • LightCycler 480II
(Roche)
의 이미지

    LightCycler 480II
    (Roche)

  • ABI7500
(Thermo Fisher Scientific)
의 이미지

    ABI7500
    (Thermo Fisher Scientific)

  • Rotor-Gene Q
(Qiagen)
의 이미지

    Rotor-Gene Q
    (Qiagen)

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