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Oncology - Tissue biopsy

PNAClamp™ Mutation Detection Kit NRAS

NRAS mutation is found in several cancers such as melanoma (13~25%), colorectal cancer (1~6%), lung cancer (1%), thyroid cancer (7%) and hepatocellular carcinoma (10%). It is know that the drug response against colorectal cancer medicine such as Erbitux and Cetuximab decreased and the prognosis of the metastatic colorectal cancer patient is bad if the patient has NRAS mutation.
CAT NO.
PNAC-1101

DETAILS

 

 

 

  
 

Total 43 NRAS mutations can be detected.

 

 Mutations

Size 

 Codon 12

Codon 13 

Codon 59 

Codon 61 

Codon 117 

Codon 146 

25 tests 

 9

 10

 3

 12

 4

 5

 

FEATURES

  • 01High sensitivity and specificity even with small amount of DNA (2% LOD with 10 ng wild/mutant type mixed DNA)
  • 02Real-Time PCR based ready-to-use kit
  • 03Short running time (within 3 hours)

PROCEDURE

  • sample preparation
  • Extraction of Total DNA
  • Amplification of target DNA by Real-Time PCR
  • Analysis of Result

COMPATIBLE SAMPE TYPE

- FFPE tissue

COMPATIBLE Real-Time PCR machine

  • CFX96
(Bio-Rad)
의 이미지

    CFX96
    (Bio-Rad)

  • LightCycler 480II
(Roche)
의 이미지

    LightCycler 480II
    (Roche)

  • ABI7500
(Thermo Fisher Scientific)
의 이미지

    ABI7500
    (Thermo Fisher Scientific)

  • ABI7900HT
(Thermo Fisher Scientific)
의 이미지

    ABI7900HT
    (Thermo Fisher Scientific)

  • StepOnePlus
(Thermo Fisher Scientific)
의 이미지

    StepOnePlus
    (Thermo Fisher Scientific)

  • Rotor-Gene Q
(Qiagen)
의 이미지

    Rotor-Gene Q
    (Qiagen)

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