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Oncology - Tissue biopsy
PNAClamp™ Mutation Detection Kit NRAS
NRAS mutation is found in several cancers such as melanoma (13~25%), colorectal cancer (1~6%), lung cancer (1%), thyroid cancer (7%) and hepatocellular carcinoma (10%). It is know that the drug response against colorectal cancer medicine such as Erbitux and Cetuximab decreased and the prognosis of the metastatic colorectal cancer patient is bad if the patient has NRAS mutation.
CAT NO.
PNAC-1101
DETAILS
Total 43 NRAS mutations can be detected.
Mutations | Size | |||||
Codon 12 | Codon 13 | Codon 59 | Codon 61 | Codon 117 | Codon 146 | 25 tests |
9 | 10 | 3 | 12 | 4 | 5 |
FEATURES
- 01High sensitivity and specificity even with small amount of DNA (2% LOD with 10 ng wild/mutant type mixed DNA)
- 02Real-Time PCR based ready-to-use kit
- 03Short running time (within 3 hours)
PROCEDURE
- sample preparation
- Extraction of Total DNA
- Amplification of target DNA by Real-Time PCR
- Analysis of Result
COMPATIBLE SAMPE TYPE
- FFPE tissue
COMPATIBLE Real-Time PCR machine
-
CFX96
(Bio-Rad)
-
LightCycler 480II
(Roche)
-
ABI7500
(Thermo Fisher Scientific)
-
ABI7900HT
(Thermo Fisher Scientific)
-
StepOnePlus
(Thermo Fisher Scientific)
-
Rotor-Gene Q
(Qiagen)